Anticorps Recombinant de lapin anti-INTS5

INTS5 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

230462B10

N° de cat : 83154-1-RR

Synonymes

INTS 5, Int5, 230462B10

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Galerie de données de validation

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  WB analysis using 83154-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83154-1-RR (INTS5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 83154-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using INTS5 antibody (83154-1-RR, Clone: 230462B10 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of HeLa using 83154-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug INTS5 Recombinant antibody (83154-1-RR, Clone:230462B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83154-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83154-1-RR against Human INTS5 were performed. The affinity constant is 0.968 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HT-29, cellules NIH/3T3, cellules SKOV-3
• Résultats positifs en IF/ICC: cellules U2OS,
• Résultats positifs en FC (Intra): cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83154-1-RR cible INTS5 dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: INTS5 Protéine recombinante Ag5129
• Nom complet: integrator complex subunit 5
• Masse moléculaire calculée: 108 kDa
• Poids moléculaire observé: 108 kDa
• Numéro d’acquisition GenBank: BC060841
• Symbole du gène: INTS5
• Identification du gène (NCBI): 80789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

INTS5, also named as INT5 and KIAA1698, is a component of the Integrator complex which involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes.

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