Anticorps Monoclonal anti-IPO4

IPO4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2F3H2

N° de cat : 67549-1-PBS

Synonymes

2F3H2, Imp4b, importin 4, Importin 4b, Importin-4

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Galerie de données de validation

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  WB analysis using 67549-1-Ig (same clone as 67549-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67549-1-Ig (IPO4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 67549-1-Ig (same clone as 67549-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 67549-1-Ig (IPO4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 67549-1-Ig (same clone as 67549-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using IPO4 antibody (67549-1-Ig, Clone: 2F3H2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67549-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67549-1-PBS cible IPO4 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IPO4 Protéine recombinante Ag2281
• Nom complet: importin 4
• Masse moléculaire calculée: 1081 aa, 118 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC003690
• Symbole du gène: IPO4
• Identification du gène (NCBI): 79711
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Importin‐4 (IPO4) belongs to the importin β family which directs the cargo to pass through the nuclear pore complex via transient interaction with nucleoporins. IPO4 is responsible for transporting histones H3 and H4 into the nucleus for chromatin assembly. Recently IPO4 has been reported to contribute to gastric cancer progression and poor prognosis.

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