Anticorps Monoclonal anti-IPO5

IPO5 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E2B9

N° de cat : 67962-1-PBS

Synonymes

1E2B9, IMB3, Imp5, importin 5, Importin subunit beta 3

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Galerie de données de validation

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  WB analysis using 67962-1-Ig (same clone as 67962-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67962-1-Ig (IPO5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67962-1-Ig (same clone as 67962-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67962-1-Ig (IPO5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67962-1-Ig (same clone as 67962-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IPO5 antibody (67962-1-Ig, Clone: 1E2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67962-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67962-1-PBS cible IPO5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IPO5 Protéine recombinante Ag30955
• Nom complet: importin 5
• Poids moléculaire observé: 124 kDa
• Numéro d’acquisition GenBank: BC001497
• Symbole du gène: IPO5
• Identification du gène (NCBI): 3843
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IPO5 is a member of the importin beta family. Structurally, the protein adopts the shape of a right-hand solenoid and is composed of 24 HEAT repeats. IPO5 facilitates cytoplasmic polyadenylation element-binding protein CPEB3 translocation by binding to the RRM1 motif of CPEB3 in neurons. NMDAR signaling increases RanBP1 expression and reduces the level of cytoplasmic GTP-bound Ran. These changes enhance CPEB3-IPO5 interaction, which consequently accelerates the nuclear import of CPEB3 and promotes its nuclear function.

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