Anticorps Polyclonal de lapin anti-IQGAP3

• Phare
• Validé par KD/KO

IQGAP3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 25930-1-AP

Synonymes

IQGAP3

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Galerie de données de validation

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  WB analysis using 25930-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25930-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 25930-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25930-1-AP (IQGAP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 25930-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25930-1-AP (IQGAP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 25930-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25930-1-AP (IQGAP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HEK-293, cellules HepG2, cellules MCF-7
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

25930-1-AP cible IQGAP3 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IQGAP3 Protéine recombinante Ag23109
• Nom complet: IQ motif containing GTPase activating protein 3
• Masse moléculaire calculée: 1631 aa, 185 kDa
• Poids moléculaire observé: 185 kDa
• Numéro d’acquisition GenBank: BC131536
• Symbole du gène: IQGAP3
• Identification du gène (NCBI): 128239
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Cancer Lett; IQGAP3 interacts with Rad17 to recruit the Mre11-Rad50-Nbs1 complex and contributes to radioresistance in lung cancer.; Authors - Yulan Zeng
human	IHC	BMC Cancer; Comprehensive analysis of the expression and prognosis for IQ motif-containing GTPase-activating proteins in hepatocellular carcinoma; Authors - Qingqing Dai
human	WB	Biochim Biophys Acta Gen Subj; Identification of the interactome of the DP1 receptor for Prostaglandin D2: Regulation of DP1 receptor signaling and trafficking by IQGAP1.; Authors - Louis Fréchette ; KD Validated
human	WB	iScience; Oncofetal protein IGF2BP1 regulates IQGAP3 expression to maintain stem cell potential in cancer; Authors - Khine Myint
human	WB	PeerJ; IQGAP3 in clear cell renal cell carcinoma contributes to drug resistance and genome stability; Authors - Wen Li ; KD Validated
human	WB,IHC,IF	Clin Cancer Res; Infiltrative vessel co-optive growth pattern induced by IQGAP3 overexpression promotes microvascular invasion in hepatocellular carcinoma; Authors - Miaoling Tang

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