Anticorps Recombinant de lapin anti-IRAK1

IRAK1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

250691C5

N° de cat : 86068-3-RR

Synonymes

EC:2.7.11.1, Interleukin-1 receptor-associated kinase 1, IRAK, IRAK 1, IRAK-1

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Galerie de données de validation

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  WB analysis using 86068-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86068-3-RR (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 86068-3-RR

  PC-12 cells were subjected to SDS PAGE followed by western blot with 86068-3-RR (IRAK1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86068-3-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using IRAK1 antibody (86068-3-RR, Clone: 250691C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of SKOV-3 using 86068-3-RR

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using IRAK1 antibody (86068-3-RR, Clone: 250691C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 86068-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86068-3-RR against Human IRAK1 were performed. The affinity constant is 2.50 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules HEK-293, cellules Jurkat, cellules MCF-7, cellules PC-12
• Résultats positifs en IF/ICC: cellules SKOV-3, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86068-3-RR cible IRAK1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IRAK1 Protéine recombinante Ag0728
• Nom complet: interleukin-1 receptor-associated kinase 1
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 68-80 kDa
• Numéro d’acquisition GenBank: BC014963
• Symbole du gène: IRAK1
• Identification du gène (NCBI): 3654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1 receptor-associated kinases (IRAKs) are a unique family of death domain containing protein kinases that play a key role in initiating innate immune response against foreign pathogens. They are involved in Toll-like receptor (TLR) and interleukin-1 receptor (IL-1R) signaling pathways. IRAK1 is the first member of this kinase family. Upon ligand binding to TLR/IL-1R, IRAK1 is recruited by MYD88 to the receptor-signaling complex, the association leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Hyper-phosphorylated IRAK1 then disengages from the receptor complex, and forms a cytosolic IRAK1-TRAF6 complex. TRAF6 then interacts with TAK and TAB, resulting in eventual activation of the NF-κB and MAPK pathways. Phosphorylated IRAK1 also undergoes ubiquitin-mediated degradation or sumoylation, which results in nuclear translocation and transcriptional activation of inflammatory target genes. (PMID: 17890055; 12620219)

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