Anticorps Recombinant de lapin anti-IRAK3

IRAK3 Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240975C5

N° de cat : 83876-2-PBS

Synonymes

240975C5, ASRT5, IL-1 receptor-associated kinase M, Inactive IL-1 receptor-associated kinase 3, Interleukin-1 receptor-associated kinase 3

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Galerie de données de validation

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  WB analysis of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 83876-2-RR (same clone as 83876-2-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 83876-2-RR (same clone as 83876-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83876-2-RR (IRAK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

• 

  IF Staining of THP-1 using 83876-2-RR (same clone as 83876-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IRAK3 antibody (83876-2-RR, Clone: 240975C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83876-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83876-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83876-2-PBS against Human IRAK3 was performed. The affinity constant is below 1 pM.

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  Affinity and Kinetic Characterization of 83876-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83876-2-RR against Human IRAK3 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

83876-2-PBS cible IRAK3 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IRAK3 Protéine recombinante Ag4959
• Nom complet: interleukin-1 receptor-associated kinase 3
• Masse moléculaire calculée: 68 kDa
• Poids moléculaire observé: 68 kDa
• Numéro d’acquisition GenBank: BC057800
• Symbole du gène: IRAK3
• Identification du gène (NCBI): 11213
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IRAK3 (Interleukin-1 receptor-associated kinase-3, also named IRAKM) as a kinase-deficient member of the TLR/IRAK family has been shown to negatively regulate Toll-like receptor (TLR)-mediated cell signaling (PMID: 33590831).

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