Anticorps Polyclonal de lapin anti-IRAK4

IRAK4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 18221-1-AP

Synonymes

IPD1, IRAK 4, IRAK4, NY REN 64, REN64

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Galerie de données de validation

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  WB analysis of Jurkat using 18221-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18221-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 18221-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 18221-1-AP (IRAK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 18221-1-AP

  IP result of anti-IRAK4 (IP:18221-1-AP, 4ug; Detection:18221-1-AP 1:300) with K-562 cells lysate 4000ug.

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  IHC staining of human lung cancer using 18221-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18221-1-AP (IRAK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18221-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18221-1-AP (IRAK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du poumon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

18221-1-AP cible IRAK4 dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRAK4 Protéine recombinante Ag12982
• Nom complet: interleukin-1 receptor-associated kinase 4
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC013316
• Symbole du gène: IRAK4
• Identification du gène (NCBI): 51135
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Integrated characterization of hepatobiliary tumor organoids provides a potential landscape of pharmacogenomic interactions; Authors - Yanjing Zhu
	WB	Mol Neurobiol; Let-7b-TLR7 Signaling Axis Contributes to the Anesthesia/Surgery-Induced Cognitive Impairment; Authors - Liyun Deng
	WB	Front Pharmacol; Euphorbia Factor L2 ameliorates the Progression of K/BxN Serum-Induced Arthritis by Blocking TLR7 Mediated IRAK4/IKKβ/IRF5 and NF-kB Signaling Pathways.; Authors - Jing Tang
sheep	WB	Mediators Inflamm; Capsular Polysaccharide is a Main Component of Mycoplasma ovipneumoniae in the Pathogen-Induced Toll-Like Receptor-Mediated Inflammatory Responses in Sheep Airway Epithelial Cells.; Authors - Zhongjia Jiang
	WB	Evid Based Complement Alternat Med; Extracts of Salvia-Nelumbinis Naturalis Ameliorate Nonalcoholic Steatohepatitis via Inhibiting Gut-Derived Endotoxin Mediated TLR4/NF-κB Activation.; Authors - Xiangbing Shu
human	WB	Int Immunopharmacol; Herbal compound cepharanthine attenuates inflammatory arthritis by blocking macrophage M1 polarization; Authors - Chenyang Lu

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