Anticorps Recombinant de lapin anti-IRE1/ERN1

IRE1/ERN1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250145G7

N° de cat : 82687-5-RR

Synonymes

ERN1, IRE1; ERN1, Endoplasmic reticulum-to-nucleus signaling 1, Endoribonuclease, ERN 1

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Galerie de données de validation

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  WB analysis using 82687-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82687-5-RR (IRE1; ERN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 82687-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82687-5-RR (IRE1; ERN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82687-5-RR

  Biolayer interferometry (BLl) kinetic assays of 82687-5-RR against Human IRE1/ERN1 were performed. The affinity constant is 1.12 nM.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A549, cellules C2C12, tissu hépatique de rat

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82687-5-RR cible IRE1/ERN1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IRE1/ERN1 Protéine recombinante Ag25580
• Nom complet: endoplasmic reticulum to nucleus signaling 1
• Masse moléculaire calculée: 977 aa, 110 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC130405
• Symbole du gène: IRE1
• Identification du gène (NCBI): 2081
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Inositol-requiring enzyme 1 (IRE1) is an ER transmembrane sensor that activates the UPR to maintain the ER and cellular function. Although mammalian IRE1 promotes cell survival, it can initiate apoptosis via decay of antiapoptotic miRNAs. IRE1 contains a ribonuclease domain in its cytoplasmic region which initiates splicing reaction by direct cleavage of XBP1 mRNA at the two stem loop structures. IRE1 plays instrumental protumoral roles in several cancers, and high IRE1 activity has been associated with poorer prognoses. (PMID: 23880584, PMID: 32861679, PMID: 31875595)

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