Anticorps Polyclonal de lapin anti-IREB2

IREB2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29976-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29976-1-AP (same clone as 29976-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29976-1-AP (IREB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 29976-1-AP (same clone as 29976-1-PBS)

  IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:5000) with mouse liver tissue lysate 1840 ug. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IP experiment of A549 using 29976-1-AP (same clone as 29976-1-PBS)

  IP result of anti-IREB2 (IP:29976-1-AP, 4ug; Detection:29976-1-AP 1:1000) with A549 cells lysate 920 ug. This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29976-1-AP (same clone as 29976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29976-1-AP (same clone as 29976-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29976-1-AP (IREB2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 29976-1-AP (same clone as 29976-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using IREB2 antibody (29976-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29976-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29976-1-PBS cible IREB2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IREB2 Protéine recombinante Ag32233
• Nom complet: iron-responsive element binding protein 2
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 105 kDa
• Numéro d’acquisition GenBank: BC017880
• Symbole du gène: IREB2
• Identification du gène (NCBI): 3658
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IREB2 is an RNA-binding protein that acts to regulate iron levels in the cells by regulating the translation and stability of mRNAs that affect iron homeostasis under conditions when iron is depleted. When iron levels are low, this protein binds to iron-responsive elements (IRES), stem-loop structures located either in the 5' or 3' UTRs. Binding to the 5' UTR represses translation, while binding to the 3' UTR inhibits mRNA degradation. When iron is found in the cell, this protein is degraded in a F-box and leucine rich repeat protein 5-dependent manner.

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