Anticorps Polyclonal de lapin anti-IRF2BP1

IRF2BP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 13698-1-AP

Synonymes

EC:2.3.2.27, Interferon regulatory factor 2-binding protein 1, IRF 2 binding protein 1, IRF 2BP1, IRF-2-binding protein 1

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Galerie de données de validation

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  WB analysis of HeLa using 13698-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lymph using 13698-1-AP

  rat lymph tissue were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13698-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13698-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 13698-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13698-1-AP (IRF2BP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 13698-1-AP

  IP result of anti-IRF2BP1 (IP:13698-1-AP, 4ug; Detection:13698-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human breast cancer using 13698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 13698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 13698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13698-1-AP (IRF2BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13698-1-AP

  Immunofluorescent analysis of Hela cells, using IRF2BP1 antibody 13698-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules Jurkat, tissu cérébral de souris, tissu lymphatique de rat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

13698-1-AP cible IRF2BP1 dans les applications de WB, IHC, IF/ICC, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRF2BP1 Protéine recombinante Ag4596
• Nom complet: interferon regulatory factor 2 binding protein 1
• Masse moléculaire calculée: 584 aa, 62 kDa
• Poids moléculaire observé: 62-72 kDa
• Numéro d’acquisition GenBank: BC038222
• Symbole du gène: IRF2BP1
• Identification du gène (NCBI): 26145
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF2BP1 inhibits IRF2-mediated transcriptional activation by interacting with IRF2. In epidermal growth factor receptor (EGFR) signaling, transient deSUMOylation of IRF2BP1 is essential for the proper expression of immediate early genes such as DUSP1 and ATF3. IRF2BP1 is also involved in the TGF-β signaling pathway by controlling the accumulation of cPML in the cytoplasm, which in turn affects the phosphorylation of Smad2 and the assembly of the Smad2-receptor complex.

Publications

Species	Application	Title
human	IF,ChIP	EMBO Rep; Transient deSUMOylation of IRF2BP proteins controls early transcription in EGFR signaling.; Authors - Sina V Barysch
human	WB	Cell Death Dis; The Sumo proteome of proliferating and neuronal-differentiating cells reveals Utf1 among key Sumo targets involved in neurogenesis.; Authors - Juan F Correa-Vázquez

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