Anticorps Polyclonal de lapin anti-IRF3

• Phare
• Validé par KD/KO

IRF3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11312-1-PBS

Synonymes

interferon regulatory factor 3, IRF 3, IRF-3

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Galerie de données de validation

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  WB analysis of Jurkat cells using 11312-1-AP (same clone as 11312-1-PBS)

  WB result of IRF3 specific antibody (11312-1-AP, 1:5000) with si-control and si-IRF3 transfected Jurkat cells. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

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  WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

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  WB analysis using 11312-1-AP (same clone as 11312-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11312-1-AP (IRF3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 11312-1-AP (same clone as 11312-1-PBS)

  IP result of anti-IRF3 (IP:11312-1-AP, 3ug; Detection:11312-1-AP 1:1000) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 11312-1-AP (same clone as 11312-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 11312-1-AP (IRF3 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 11312-1-AP (same clone as 11312-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IRF3 antibody (11312-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 11312-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11312-1-PBS cible IRF3 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRF3 Protéine recombinante Ag1858
• Nom complet: interferon regulatory factor 3
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC009395
• Symbole du gène: IRF3
• Identification du gène (NCBI): 3661
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa.

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