Anticorps Monoclonal anti-IRF3

IRF3 Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1E6G8

N° de cat : 66670-1-Ig

Synonymes

interferon regulatory factor 3, IRF 3, IRF3

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Galerie de données de validation

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  WB analysis using 66670-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 66670-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 66670-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66670-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 66670-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 66670-1-Ig

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Daudi, cellules HepG2, cellules Jurkat, cellules MOLT-4, cellules THP-1
• Résultats positifs en IHC: tissu splénique humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

66670-1-Ig cible IRF3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IRF3 Protéine recombinante Ag27223
• Nom complet: interferon regulatory factor 3
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC009395
• Symbole du gène: IRF3
• Identification du gène (NCBI): 3661
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.

Publications

Species	Application	Title
human	WB,IF	Front Immunol; Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1.; Authors - Zhihai Zhou
human	WB	Vet Microbiol; DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocation; Authors - Shujuan Xu
human,mouse	WB	mBio; MIR155HG Plays a Bivalent Role in Regulating Innate Antiviral Immunity by Encoding Long Noncoding RNA-155 and microRNA-155-5p; Authors - Kul Raj Rai
human	WB	Cell Commun Signal; Bacterial vesicles block viral replication in macrophages via TLR4-TRIF-axis; Authors - Jeff Bierwagen
human	WB	J Med Virol; SADS-CoV nsp1 inhibits the IFN-β production by preventing TBK1 phosphorylation and inducing CBP degradation; Authors - Yingjie Xiang
mouse	WB	Autophagy; CKLF induces microglial activation via triggering defective mitophagy and mitochondrial dysfunction; Authors - Hongyun Wang