Anticorps Monoclonal anti-IRF3

IRF3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1E6G8

N° de cat : 66670-1-PBS

Synonymes

1E6G8, interferon regulatory factor 3, IRF 3, IRF-3

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Galerie de données de validation

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  WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66670-1-Ig (same clone as 66670-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66670-1-Ig (same clone as 66670-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66670-1-Ig (IRF3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66670-1-Ig (same clone as 66670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66670-1-Ig (same clone as 66670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66670-1-Ig (same clone as 66670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 66670-1-Ig (same clone as 66670-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66670-1-Ig (IRF3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66670-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66670-1-PBS cible IRF3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IRF3 Protéine recombinante Ag27223
• Nom complet: interferon regulatory factor 3
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 50-60 kDa
• Numéro d’acquisition GenBank: BC009395
• Symbole du gène: IRF3
• Identification du gène (NCBI): 3661
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.

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