Anticorps Monoclonal anti-IRF5

IRF5 Monoclonal Antibody for WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB,ELISA

Conjugaison

Non conjugué

CloneNo.

3A4A10

N° de cat : 66835-1-Ig

Synonymes

interferon regulatory factor 5, IRF 5, IRF5, SLEB10

---

Galerie de données de validation

• 

  WB analysis of A549 using 66835-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 66835-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 66835-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66835-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66835-1-Ig (IRF5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules RAW 264.7, cellules THP-1

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66835-1-Ig cible IRF5 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IRF5 Protéine recombinante Ag5442
• Nom complet: interferon regulatory factor 5
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC004201
• Symbole du gène: IRF5
• Identification du gène (NCBI): 3663
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF5, also named as SLEB10, contians one IRF tryptophan pentad repeat DNA-binding domain and belongs to the IRF family. It is a transcription factor involved in the induction of interferons IFNA and INFB and inflammatory cytokines upon virus infection. It is activated by TLR7 or TLR8 signaling. Genetic variations in IRF5 are associated with susceptibility to inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). IRF5 is also used as M1 marcrophage lineage marker. Alternative splice variant encoding different isoforms exist.