Anticorps Polyclonal de lapin anti-IRF7

IRF7 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 22392-1-AP

Synonymes

IRF 7, IRF 7H, IRF7, IRF7A

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Galerie de données de validation

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  WB analysis of HEK-293 using 22392-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22392-1-AP (IRF7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 22392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 22392-1-AP (IRF7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 22392-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 22392-1-AP (IRF7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293,
• Résultats positifs en IHC: tissu cutané de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

22392-1-AP cible IRF7 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRF7 Protéine recombinante Ag18059
• Nom complet: IRF 7
• Masse moléculaire calculée: 516 aa, 56 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC136555
• Symbole du gène: IRF7
• Identification du gène (NCBI): 3665
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF-7 (IFN regulatory factor 7) is a member of the IFN regulatory transcription factor (IRF) family. IRF-7 has been shown to play a role in the transcriptional activation of virus-inducible cellular proteins, including IFN beta chain proteins. Inducible expression of IRF-7 is largely restricted to lymphoid tissue. Four transcript variants encoding distinct isoforms (A,B,C and D) have been identified for this (PMID:9786932).The active IRF7A exists as a dimer form ~80 kDa(PMID:11073981). The MW 67-70 kDa has been reported in some papers (PMID:9786932; 22951831).Various posttranslational modifications of IRF7 including phosphorylation, ubiquitination, sumoylation and acetylation are identified (PMID:22951831).This antibody is a rabbit polyclonal antibody raised against the C-terminal 349 amino acid residues of human IRF7 D. The molecular weight of Nonphosphorylated IRF7 cofractionated with the 44-kDa marker, approximating its predicted size. In contrast, phosphorylated IRF7, which migrate more slowly on SDS-PAGE. This phosphorylated IRF7 was consistent with a size of 80 to 90 kDa. (PMID: 11073981 )

Publications

Species	Application	Title
human	WB	Cell Chem Biol; Identifying enhancers of innate immune signaling as broad-spectrum antivirals active against emerging viruses.; Authors - Ghizlane Maarifi
human	WB	J Immunol; Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.; Authors - Zhangchuan Xia
human	WB	J Immunol; Inducible ATP1B1 Upregulates Antiviral Innate Immune Responses by the Ubiquitination of TRAF3 and TRAF6.; Authors - Wei Cao
mouse	WB	Phytomedicine; Si-Ni-San ameliorates chronic colitis by modulating type I interferons-mediated inflammation.; Authors - Yajie Cai
human	WB	Cancer Med; A regulatory mutant on TRIM26 conferring the risk of nasopharyngeal carcinoma by inducing low immune response.; Authors - Xiao-Ming Lyu
human	WB	Mol Med Rep; TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes.; Authors - Xinxin Liu