Anticorps Polyclonal de lapin anti-IRF9

• Phare
• Validé par KD/KO

IRF9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

22

N° de cat : 14167-1-AP

Synonymes

IRF 9, IRF9, ISGF 3 gamma, ISGF3, ISGF3 p48 subunit, ISGF3G, p48

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Galerie de données de validation

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  WB analysis using 14167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14167-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14167-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 14167-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14167-1-AP (IRF9 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 14167-1-AP

  IP Result of anti-IRF9 (IP:14167-1-AP, 4ug; Detection:14167-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human cervical cancer using 14167-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14167-1-AP (IRF9 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 14167-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14167-1-AP (IRF9 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14167-1-AP

  Immunofluorescent analysis of HepG2 cells, using IRF9 antibody 14167-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules MCF-7, cellules THP-1, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

14167-1-AP cible IRF9 dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRF9 Protéine recombinante Ag5365
• Nom complet: IRF 9
• Masse moléculaire calculée: 393 aa, 44 kDa
• Poids moléculaire observé: 44-48 kDa
• Numéro d’acquisition GenBank: BC035716
• Symbole du gène: IRF9
• Identification du gène (NCBI): 10379
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
mouse	WB	Cell Rep; miR-342-5p decreases ankyrin G levels in Alzheimer's disease transgenic mouse models.; Authors - Xiaqin Sun
	ChIP	PLoS Pathog; A host cell long noncoding RNA NR_033736 regulates type I interferon-mediated gene transcription and modulates intestinal epithelial anti-Cryptosporidium defense.; Authors - Juan Li
		Front Immunol; PMID: 30158934; Authors - Ming-Hsun Tsai
human	WB	Mol Cancer Ther; JAK2 inhibitor SAR302503 abrogates PD-L1 expression and targets therapy resistant non-small cell lung cancers.; Authors - Sean Pitroda
human	WB, IP, IF	J Immunol; Herpes Simplex Virus Type 2 Inhibits Type I IFN Signaling Mediated by the Novel E3 Ubiquitin Protein Ligase Activity of Viral Protein ICP22.; Authors - Mudan Zhang