Anticorps Recombinant de lapin anti-IRF9

IRF9 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242772F12

N° de cat : 85160-2-PBS

Synonymes

242772F12, IFN-alpha-responsive transcription factor subunit, Interferon-stimulated gene factor 3 gamma, IRF 9, IRF-9

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Galerie de données de validation

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  WB analysis using 85160-2-RR (same clone as 85160-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85160-2-RR (IRF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85160-2-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 85160-2-RR (same clone as 85160-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85160-2-RR (IRF9 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85160-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85160-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85160-2-RR against Human IRF9 were performed. The affinity constant is 0.136 nM.

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Informations sur le produit

85160-2-PBS cible IRF9 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IRF9 Protéine recombinante Ag5365
• Nom complet: IRF 9
• Masse moléculaire calculée: 393 aa, 44 kDa
• Poids moléculaire observé: 44-48 kDa
• Numéro d’acquisition GenBank: BC035716
• Symbole du gène: IRF9
• Identification du gène (NCBI): 10379
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IRF9 also named ISGF3 is a transcription regulatory factor that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with IRF9/ISGF3G to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN stimulated genes, which drive the cell in an antiviral state.

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