Anticorps Recombinant de lapin anti-IRG1

IRG1 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

251531C12

N° de cat : 86725-1-RR

Synonymes

ACOD1, CAD

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Galerie de données de validation

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  WB analysis of RAW 264.7 using 86725-1-RR

  LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 86725-1-RR (IRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 86725-1-RR

  IFN gamma and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 86725-1-RR (IRG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of RAW 264.7 using 86725-1-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS treated RAW 264.7 cells using IRG1 antibody (86725-1-RR, Clone: 251531C12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7 traitées au LPS, IFN gamma and LPS treated THP-1 cells
• Résultats positifs en IF/ICC: cellules RAW 264.7 traitées au LPS,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86725-1-RR cible IRG1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IRG1 Protéine recombinante Ag28913
• Nom complet: immunoresponsive 1 homolog (mouse)
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: NM_001258406
• Symbole du gène: IRG1
• Identification du gène (NCBI): 730249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The host Immune-Responsive Gene 1 (IRG1; also called Acod1) is a mitochondrial enzyme induced under inflammatory conditions that produces the metabolite itaconate by decarboxylating cis-aconitate, a TCA cycle intermediate. Gene expression profiling studies of murine macrophages and microglial cells have revealed that IRG1 is highly expressed under pro-inflammatory conditions. Furthermore, IRG1 is highly expressed in the pregnant uterus during the early events leading to implantation, the specific phase of pregnancy in which high levels of inflammatory cytokines are secreted. IRG1 localizes to the mitochondria and may represent a key link between immunological and metabolic processes. IRG1 has crucial functions in embryonic implantation and neurodegeneration. Also, IRG1 promotes endotoxin tolerance by increasing A20 expression in macrophages via increased ROS production. (PMID: 25640654)

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