Anticorps Polyclonal de lapin anti-IRS1

• Phare
• Validé par KD/KO

IRS1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (4)

Applications

WB, IP, IHC, IF, FC, Dot blot, ELISA

Conjugaison

Non conjugué

Publications

52

N° de cat : 17509-1-AP

Synonymes

HIRS 1, IRS 1, IRS1, IRS-1

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Galerie de données de validation

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  WB analysis of A549 using 17509-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 17509-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 17509-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17509-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 17509-1-AP

  IP Result of anti-IRS1 (IP:17509-1-AP, 5ug; Detection:17509-1-AP 1:1000) with A549 cells lysate 3500ug.

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  IHC staining of human breast cancer using 17509-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 17509-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 17509-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 17509-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A549 using 17509-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using IRS1 antibody (17509-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of A549 using 17509-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using IRS1 antibody (17509-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  FC experiment of MCF-7 using 17509-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human IRS1 (17509-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules A431, cellules HEK-293, cellules PC-3
• Résultats positifs en IP: cellules A549
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A549,
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 50 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

17509-1-AP cible IRS1 dans les applications de WB, IP, IHC, IF, FC, Dot blot, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris, Megalobrama Amblycephala
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IRS1 Protéine recombinante Ag11714
• Nom complet: insulin receptor substrate 1
• Masse moléculaire calculée: 1242 aa, 132 kDa
• Poids moléculaire observé: 160-185 kDa
• Numéro d’acquisition GenBank: BC053895
• Symbole du gène: IRS1
• Identification du gène (NCBI): 3667
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.

Publications

Species	Application	Title
human	WB	Mol Cell; KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis; Authors - Hiroshi Senoo
mouse	WB	Autophagy; BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.; Authors - Simone C da Silva Rosa
mouse	Dot blot	Environ Health Perspect; Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice.; Authors - Jacob Raber
mouse	WB	JHEP Rep; Human umbilical cord mesenchymal stem cell-derived exosomes ameliorate liver steatosis by promoting fatty acid oxidation and reducing fatty acid synthesis; Authors - Fuji Yang
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
mouse	WB	Elife; EPHX1 mutations cause a lipoatrophic diabetes syndrome due to impaired epoxide hydrolysis and increased cellular senescence.; Authors - Jeremie Gautheron