Anticorps Polyclonal de lapin anti-IRS2

IRS2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20702-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 20702-1-AP (same clone as 20702-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20702-1-AP (IRS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20702-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20702-1-AP (same clone as 20702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20702-1-AP (IRS2 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 20702-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 20702-1-AP (same clone as 20702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20702-1-AP (IRS2 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 20702-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20702-1-PBS cible IRS2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: IRS2
• Masse moléculaire calculée: 137 kDa
• Poids moléculaire observé: 150-185 kDa
• Numéro d’acquisition GenBank: NM_003749
• Symbole du gène: IRS2
• Identification du gène (NCBI): 8660
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The family of INS receptor substrates (IRS) consists of four proteins (IRS-1-IRS-4), which were initially characterized as typical cytosolic adaptor proteins involved in INS receptor (IR) and INS-like growth factor I receptor (IGF-IR) signaling. Impaired glucose tolerance and INS resistance, and thus type 2 diabetes were demonstrated in Irs2 knockout mice. In vivo data also indicated that he regulation of beta-cell mass involves IRS2, while IRS1 is only required for proper INS production in beta-cells. IRS2, like IRS1, has a predicted molecular weight of 137 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS-PAGE as a band of approximately 150-185 kDa.

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