Anticorps Monoclonal anti-ISG15

ISG15 Monoclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3G9A2

N° de cat : 68408-1-PBS

Synonymes

G1P2, hUCRP, IFI15, IP17, ISG15, ISG15 ubiquitin like modifier, Ubiquitin like protein ISG15, UCRP

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Galerie de données de validation

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  WB analysis using 68408-1-Ig (same clone as 68408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 68408-1-Ig (same clone as 68408-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68408-1-Ig (same clone as 68408-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68408-1-Ig (ISG15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68408-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68408-1-PBS cible ISG15 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ISG15 Protéine recombinante Ag8953
• Nom complet: ISG15 ubiquitin-like modifier
• Masse moléculaire calculée: 165 aa, 18 kDa
• Poids moléculaire observé: 13-17 kDa
• Numéro d’acquisition GenBank: BC009507
• Symbole du gène: ISG15
• Identification du gène (NCBI): 9636
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ISG15 is a ubiquitin-like protein that becomes conjugated to many cellular proteins upon activation by interferon-alpha (IFNA) and -beta (IFNB). ISG15 forms covalent conjugates with its target proteins in a process called ISGylation, which in mammals is known to play a role in antiviral immunity. ISG15 proteins possess two ubiquitin-like (UBL) domains and a highly conserved C-terminal LRGG sequence, the latter being known as the ubiquitin conjugation motif. Intracellular ISG15 are conjugated, via the LRGG motif, to target proteins through a process called ISGylation, which resembles largely ubiquitination, the process of formation of ubiquitin conjugates. Unconjugated extracellular ISG15, which are released from several types of human and murine cells, are known to possess cytokine-like activity.

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