Anticorps Polyclonal de lapin anti-ISG20L2

ISG20L2 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 24639-1-AP

Synonymes

EC:3.1.-.-, HSD-38, Interferon-stimulated 20 kDa exonuclease-like 2

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Galerie de données de validation

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  WB analysis of HEK-293 using 24639-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24639-1-AP (ISG20L2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24639-1-AP

  IP result of anti-ISG20L2 (IP:24639-1-AP, 4ug; Detection:24639-1-AP 1:1000) with HEK-293 cells lysate 3240ug.

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Applications testées

• Résultats positifs en WB: cellules HEK-293,
• Résultats positifs en IP: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

24639-1-AP cible ISG20L2 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ISG20L2 Protéine recombinante Ag20026
• Nom complet: interferon stimulated exonuclease gene 20kDa-like 2
• Masse moléculaire calculée: 353 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC000575
• Symbole du gène: ISG20L2
• Identification du gène (NCBI): 81875
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ISG20L2, also named HSD38, is a member of a vertebrate exonucleases family. ISG20L2 is composed of at least two functional domains, one at its N-terminal half allowing intracellular localization and association with the binding partners and the other at its C-terminal half bearing the 3` to 5` exoribonuclease activity. The molecular weight of ISG20L2 is 39 kDa (PMID: 18065403).

Publications

Species	Application	Title
human	WB	JCI Insight; ISG20L2 suppresses bortezomib anti-myeloma activity by attenuating bortezomib binding to PSMB5; Authors - Yan Yang
human	WB	Nucleic Acids Res; Molecular mechanism of human ISG20L2 for the ITS1 cleavage in the processing of 18S precursor ribosomal RNA; Authors - Yinliang Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sara (Verified Customer) (05-24-2023)

It is the best ISG20L2 I have tried by far compared to other commercial ones. I have used it for WB (1:5000) and for immunoprecipitation. I need to mention that we tried it for IF but it did not work properly, at least for Jurkat T cells!

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:5000

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