Anticorps Polyclonal de lapin anti-ISLR

ISLR Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 24306-1-PBS

Synonymes

HsT17563, Immunoglobulin superfamily containing leucine-rich repeat protein, UNQ189/PRO215

---

Galerie de données de validation

• 

  WB analysis using 24306-1-AP (same clone as 24306-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24306-1-AP (ISLR antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24306-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 24306-1-AP (same clone as 24306-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24306-1-AP (ISLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24306-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 24306-1-AP (same clone as 24306-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24306-1-AP (ISLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24306-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 24306-1-AP (same clone as 24306-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24306-1-AP (ISLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24306-1-PBS in a different storage buffer formulation.

• 

  IF Staining of NIH/3T3 using 24306-1-AP (same clone as 24306-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using ISLR antibody (24306-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24306-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 24306-1-AP (same clone as 24306-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using ISLR antibody (24306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24306-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 24306-1-AP (same clone as 24306-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ISLR antibody (24306-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24306-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

24306-1-PBS cible ISLR dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ISLR Protéine recombinante Ag19446
• Nom complet: immunoglobulin superfamily containing leucine-rich repeat
• Masse moléculaire calculée: 428 aa, 46 kDa
• Poids moléculaire observé: 44-46 kDa
• Numéro d’acquisition GenBank: BC022478
• Symbole du gène: ISLR
• Identification du gène (NCBI): 3671
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The Immunoglobulin superfamily containing leucine-rich repeat protein(ISLR), is involved in various biological events, such as embryonic development, Gaucher's disease, and replicative senescence of fibroblasts in some organs, including the heart, pancreas, and bone marrow. ISLR, also known as meflin, is a newly discovered marker of mesenchymal stem cells, which is involved in pathological fibrosis and the cancer microenvironment. (PMID: 34713300)

{{ptg:RelatedPrimaryAntibodies}}