Anticorps Recombinant de lapin anti-ISLR2

ISLR2 Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241227D6

N° de cat : 84076-3-PBS

Synonymes

241227D6, Immunoglobulin superfamily containing leucine-rich repeat protein 2, KIAA1465, Leucine-rich repeat domain and immunoglobulin domain-containing axon extension protein, LINX

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Galerie de données de validation

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  WB analysis using 84076-3-RR (same clone as 84076-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84076-3-RR (ISLR2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84076-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84076-3-RR (same clone as 84076-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84076-3-RR (ISLR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84076-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 84076-3-RR (same clone as 84076-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84076-3-RR (ISLR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84076-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84076-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84076-3-RR against Human ISLR2 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84076-3-PBS cible ISLR2 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: ISLR2 Protéine recombinante Ag20505
• Nom complet: immunoglobulin superfamily containing leucine-rich repeat 2
• Masse moléculaire calculée: 745 aa, 79 kDa
• Poids moléculaire observé: 79 kDa, 130 kDa
• Numéro d’acquisition GenBank: BC152429
• Symbole du gène: ISLR2
• Identification du gène (NCBI): 57611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ISLR2 (immunoglobulin superfamily containing leucine rich repeat 2), also known as LINX. It is expected to be located in cell membrane and the protein is mainly expressed in brain and testis. While the first knockout mouse had impaired development of the internal capsule and no hydrocephalus, a much more recent independent knockout mouse was shown to additionally have severe hydrocephalus. Although the mechanism remains unclear, it may be related to the role of ISLR2 in reorganizing the cytoskeleton of developing neurons (PMID: 30483960). The calculated molecular weight of ISLR2 is 79 kDa, this target has phosphorylation modification and glycosylation modification. And the 130 kDa band is glycosylated (PMID: 29739947).

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