Anticorps Polyclonal de lapin anti-ISYNA1

ISYNA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 14142-1-AP

Synonymes

Inositol-3-phosphate synthase 1, INOS, INO1, hIPS, hINO1

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Galerie de données de validation

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  WB analysis of mouse testis using 14142-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14142-1-AP (ISYNA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14142-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14142-1-AP (ISYNA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 14142-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14142-1-AP (ISYNA1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 14142-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 14142-1-AP (ISYNA1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 14142-1-AP

  Immunofluorescent analysis of HepG2 cells, using ISYNA1 antibody 14142-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HepG2, tissu testiculaire de rat
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

14142-1-AP cible ISYNA1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ISYNA1 Protéine recombinante Ag5272
• Nom complet: inositol-3-phosphate synthase 1
• Masse moléculaire calculée: 61 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC066902
• Symbole du gène: ISYNA1
• Identification du gène (NCBI): 51477
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myo-inositol 3-phosphate synthase (ISYNA1/IP synthase) is a rate-limiting enzyme that catalyzes the first step in the biosynthesis of all inositol containing compounds. It converts glucose 6-phosphate to Myo-inositol 3-phosphate.(PMID:21841945). The native enzyme is typically a homotrimermade up of 68-kDa subunits in mammalian cells and a homotetramer in yeast and plant cells. There are three new isoforms of 62, 43, and 16kDa detected suggesting ISYNA1 is a completely different holoenzyme. But only the brain and testis manifest the 68-kDa isoform, whereas the pancreas has a slightly smaller isoformat 67 kDa. The intestine has a completely unique isoformprofile comprising 62,43, 20kDa isoforms(PMID:19188364).

Publications

Species	Application	Title
mouse	WB	Acta Pharmacol Sin; Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway.; Authors - Jian-Yi Dong
mouse	WB	Front Pharmacol; Naringin Exerts Therapeutic Effects on Mice Colitis: A Study Based on Transcriptomics Combined With Functional Experiments; Authors - Jianyi Dong
		Front Pharmacol; Arbutin Ameliorates Murine Colitis by Inhibiting JAK2 Signaling Pathway; Authors - Liang Wang
rat	WB, IF	Alcohol Clin Exp Res; Ethanol supports macrophage recruitment and reinforces invasion and migration of lewis lung carcinoma.; Authors - Keke Yu
mouse	WB	Mol Reprod Dev; Comparative proteomic analysis of proteins involved in oocyte meiotic maturation in mice.; Authors - Cao Senyang S
	WB	Biomed Pharmacother; Sulforaphane decreases oxidative stress and inhibits NLRP3 inflammasome activation in a mouse model of ulcerative colitis; Authors - Zi-Juan Zhou

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