Anticorps Monoclonal anti-ITCH

ITCH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E3G9

N° de cat : 67757-1-PBS

Synonymes

AIF4, AIP4, dJ468O1.1, ITCH, NAPP1, NFE2 associated polypeptide 1

---

Galerie de données de validation

• 

  WB analysis using 67757-1-Ig (same clone as 67757-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 67757-1-Ig (same clone as 67757-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 67757-1-Ig (same clone as 67757-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67757-1-Ig (same clone as 67757-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  WB analysis of LNCaP using 67757-1-Ig (same clone as 67757-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67757-1-Ig (ITCH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 67757-1-Ig (same clone as 67757-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ITCH antibody (67757-1-Ig, Clone: 2E3G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67757-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

67757-1-PBS cible ITCH dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ITCH Protéine recombinante Ag14688
• Nom complet: itchy E3 ubiquitin protein ligase homolog (mouse)
• Masse moléculaire calculée: 903 aa, 103 kDa
• Poids moléculaire observé: 103 kDa, 70 kDa
• Numéro d’acquisition GenBank: BC011571
• Symbole du gène: ITCH
• Identification du gène (NCBI): 83737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

{{ptg:RelatedPrimaryAntibodies}}