Anticorps Polyclonal de lapin anti-Integrin alpha 1

• Phare
• Validé par KD/KO

Integrin alpha 1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(13)

Avis (1)

N° de cat : 22146-1-AP

Synonymes

ITGA1, VLA 1, Laminin and collagen receptor, integrin, alpha 1, CD49a

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Galerie de données de validation

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  WB analysis using 22146-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22146-1-AP (Integrin alpha-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22146-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22146-1-AP (ITGA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22146-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22146-1-AP (Integrin alpha-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 22146-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 22146-1-AP (ITGA1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 22146-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22146-1-AP (Integrin Alpha 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 22146-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22146-1-AP (Integrin Alpha 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 22146-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22146-1-AP (ITGA1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 22146-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 22146-1-AP (ITGA1 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HT-29, cellules SMMC-7721, cellules SW480
• Résultats positifs en IHC: tissu hépatique humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

22146-1-AP cible Integrin alpha 1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin alpha 1 Protéine recombinante Ag17584
• Nom complet: integrin, alpha 1
• Masse moléculaire calculée: 1179 aa, 131 kDa
• Poids moléculaire observé: 180 kDa
• Numéro d’acquisition GenBank: BC137121
• Symbole du gène: Integrin alpha 1
• Identification du gène (NCBI): 3672
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).

Publications

Species	Application	Title
human	WB,IHC	Mediators Inflamm; ITGA1 Promotes Glioma Cell Proliferation and Affects Immune Cell Infiltration in Low-Grade Glioma; Authors - Yanhong Ren
human	WB	Int J Biol Sci; Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer.; Authors - Hai Li ; KD Validated
human	WB	Int J Oncol; Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules; Authors - AMINA BOLATKAN
human	WB	J Ethnopharmacol; A combination index and glycoproteomics-based approach revealed synergistic anticancer effects of curcuminoids of turmeric against prostate cancer PC3 cells.; Authors - Meng-Meng Wei
human	WB,IHC,IF	Int J Oncol; FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.; Authors - Junfen Xu
rat	WB,IF	J Biomech; Integrin endocytosis on elastic substrates mediates mechanosensing.; Authors - Jing Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (04-24-2019)

This antibody labelled a band above 250 kD, which is likely the glycosylated form of Itga1. But, it did not label any band around 170 kD as shown in the data sheet.

• Applications: Western Blot,
• Cell Tissue Type: MEF

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