Anticorps Polyclonal de lapin anti-CD41/Integrin Alpha 2B

CD41/Integrin Alpha 2B Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

32

N° de cat : 24552-1-AP

Synonymes

CD41, CD41/Integrin Alpha 2B, CD41B, GP2B, GPalpha IIb, GPIIb, GTA, HPA3, Integrin alpha IIb, ITGA2B, ITGAB

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Galerie de données de validation

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  WB analysis using 24552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24552-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat blood using 24552-1-AP

  mouse spleen and rat blood were subjected to SDS PAGE followed by western blot with 24552-1-AP (CD41/Integrin alpha 2b antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of human plasma using 24552-1-AP

  IP Result of anti-CD41/Integrin alpha 2b (IP:24552-1-AP, 4ug; Detection:24552-1-AP 1:2000) with human plasma lysate 4000ug.

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  IHC staining of mouse spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 24552-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 24552-1-AP (CD41/Integrin Alpha 2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24552-1-AP (ITGA2B Antibody) at dilution of 1:50.

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  IHC staining of human spleen using 24552-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 24552-1-AP (ITGA2B Antibody) at dilution of 1:50.

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Applications testées

• Résultats positifs en WB: tissu pulmonaire de souris, sang de rat, tissu sanguin humain, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: tissu plasmatique humain
• Résultats positifs en IHC: tissu splénique de souris, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 5 publications below
• IF: See 8 publications below

Informations sur le produit

24552-1-AP cible CD41/Integrin Alpha 2B dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD41/Integrin Alpha 2B Protéine recombinante Ag19459
• Nom complet: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• Masse moléculaire calculée: 1039 aa, 113 kDa
• Poids moléculaire observé: 110-125 kDa
• Numéro d’acquisition GenBank: BC126442
• Symbole du gène: ITGA2B
• Identification du gène (NCBI): 3674
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light (25 kDa) and heavy (125 kDa) chains which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrin alpha-IIb/beta-3 is crucial for platelet aggregation through binding of soluble fibrinogen.

Publications

Species	Application	Title
human	WB	Sci Adv; Platelet-derived porous nanomotor for thrombus therapy.; Authors - Mimi Wan
human,mouse	IF	Theranostics; Platelets promote CRC by activating the C5a/C5aR1 axis via PSGL-1/JNK/STAT1 signaling in tumor-associated macrophages; Authors - Xueqin Li
human	WB	J Thromb Haemost; SINE-VNTR-Alu retrotransposon insertion as a novel mutational event underlying Glanzmann thrombasthenia; Authors - Jiasheng Zhang
mouse	IF	Theranostics; Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production.; Authors - Xin Chen
human	WB	Mol Ther; Disruption of Chromosomal Architecture of cox2 Locus Sensitizes Lung Cancer Cells to Radiotherapy.; Authors - Yuxiang Sun
human	WB	Mater Today Bio; Platelet-derived extracellular vesicles ameliorate intervertebral disc degeneration by alleviating mitochondrial dysfunction; Authors - Zhanqiu Dai

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