Anticorps Polyclonal de lapin anti-Integrin alpha-5/CD49e

• Phare
• Validé par KD/KO

Integrin alpha-5/CD49e Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(41)

Avis (1)

N° de cat : 10569-1-AP

Synonymes

Integrin Alpha-5, ITGA5, CD49 antigen-like family member E, CD49e, FNRA

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Galerie de données de validation

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  WB analysis using 10569-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10569-1-AP (Integrin Alpha-5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10569-1-AP

  WB result of Integrin alpha-5 antibody (10569-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin alpha-5 transfected HeLa cells.

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  WB analysis of human placenta using 10569-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COS-7 using 10569-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 10569-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10569-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10569-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10569-1-AP (ITGA5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 10569-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10569-1-AP (Integrin alpha-5 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10569-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10569-1-AP (Integrin alpha-5 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 10569-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10569-1-AP (Integrin Alpha-5 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules 3T3-L1, cellules COS-7, cellules HeLa, tissu placentaire humain
• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer de la peau humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 30 publications below
• IHC: See 2 publications below
• IF: See 10 publications below

Informations sur le produit

10569-1-AP cible Integrin alpha-5/CD49e dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, singe, souris

• Réactivité: Humain, singe, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin alpha-5/CD49e Protéine recombinante Ag0860
• Nom complet: integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
• Masse moléculaire calculée: 115 kDa
• Poids moléculaire observé: 135-150 kDa
• Numéro d’acquisition GenBank: BC008786
• Symbole du gène: Integrin alpha 5
• Identification du gène (NCBI): 3678
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).

Publications

Species	Application	Title
		Biomaterials; Osteogenesis potential of different titania nanotubes in oxidative stress microenvironment.; Authors - Yonglin Yu
mouse	WB	Int J Mol Sci; Atp6v1h Deficiency Blocks Bone Loss in Simulated Microgravity Mice through the Fos-Jun-Src-Integrin Pathway; Authors - Zanyan Zhao
human	WB,IF	Front Cell Dev Biol; Mesenchymal Stem Cells With Cancer-Associated Fibroblast-Like Phenotype Stimulate SDF-1/CXCR4 Axis to Enhance the Growth and Invasion of B-Cell Acute Lymphoblastic Leukemia Cells Through Cell-to-Cell Communication.; Authors - Chengyun Pan
human	WB	Oncotarget; Annexin A4 fucosylation enhances its interaction with the NF-kB p50 and promotes tumor progression of ovarian clear cell carcinoma.; Authors - Huimin Wang
		Sci Rep; Evidence of novel miR-34a-based therapeutic approaches for multiple myeloma treatment.; Authors - Mayra Rachele Zarone
human	IF	Front Oncol; ITGA5 Is a Novel Oncogenic Biomarker and Correlates With Tumor Immune Microenvironment in Gliomas.; Authors - Shuyu Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (02-04-2019)

Excellent. In WB, it gives a protein band at the expected molecular weight, and this band was decreased during adipocyte differentiation, which is consistent with those reported in literature. In IF, it labels endogenous protein at the cell surface, as that reported in literature.

• Applications: Western Blot, Immunofluorescence,

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