Anticorps Polyclonal de lapin anti-CD11c/Integrin Alpha X

CD11c/Integrin Alpha X Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

51

N° de cat : 17342-1-AP

Synonymes

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6

---

Galerie de données de validation

• 

  WB analysis of human plasma using 17342-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17342-1-AP (ITGAX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17342-1-AP (CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17342-1-AP (CD11c/Integrin alpha X Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HL-60 using 17342-1-AP

  1X10^6 HL-60 cells were stained with 0.2ug CD11c/Integrin alpha X antibody (17342-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

---

Applications testées

• Résultats positifs en WB: tissu sanguin humain
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HL-60

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below
• IHC: See 22 publications below
• IF: See 15 publications below
• FC: See 4 publications below

Informations sur le produit

17342-1-AP cible CD11c/Integrin Alpha X dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD11c/Integrin Alpha X Protéine recombinante Ag11380
• Nom complet: integrin, alpha X (complement component 3 receptor 4 subunit)
• Masse moléculaire calculée: 1169 aa, 129 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC038237
• Symbole du gène: ITGAX
• Identification du gène (NCBI): 3687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Publications

Species	Application	Title
human	IHC	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
	IHC	Acta Pharm Sin B; Celastrol targets adenylyl cyclase-associated protein 1 to reduce macrophages-mediated inflammation and ameliorates high fat diet-induced metabolic syndrome in mice.; Authors - Yuyu Zhu
	FC	Biomaterials; Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.; Authors - Haoran Wang
human	IF	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
	FC	Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang
	IF	Theranostics; Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.; Authors - Ke Feng

Produits associés

CD11c/Integrin Alpha X Monoclonal antibody

60258-1-Ig

CD11B/Integrin Alpha M Polyclonal antibody

20991-1-AP

CD11a/Integrin Alpha L Monoclonal antibody

66256-1-Ig

CD68 Polyclonal antibody

25747-1-AP

CD68 Monoclonal antibody

66231-1-Ig

CD14 Polyclonal antibody

17000-1-AP

CD14 Monoclonal antibody

60253-1-Ig