Anticorps Polyclonal de lapin anti-CD11c

CD11c Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(69)

N° de cat : 17342-1-AP

Synonymes

CD11 antigen-like family member C, Integrin alpha X, Integrin alpha-X, ITGAX, Leu M5

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Galerie de données de validation

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  WB analysis using 17342-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 17342-1-AP (CD11c/Integrin Alpha X antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 17342-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 17342-1-AP (ITGAX antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin Alpha X antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 17342-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17342-1-AP (CD11c/Integrin Alpha X antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules THP-1, plasma humain
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 30 publications below
• IF: See 23 publications below

Informations sur le produit

17342-1-AP cible CD11c dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD11c Protéine recombinante Ag11380
• Nom complet: integrin, alpha X (complement component 3 receptor 4 subunit)
• Masse moléculaire calculée: 1169 aa, 129 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC038237
• Symbole du gène: CD11c
• Identification du gène (NCBI): 3687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Publications

Species	Application	Title
human	IHC	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
	IF	Adv Sci (Weinh); Bioactive Glycyrrhizic Acid Ionic Liquid Self-Assembled Nanomicelles for Enhanced Transdermal Delivery of Anti-Photoaging Signal Peptides; Authors - Zhuxian Wang
	IHC	Acta Pharm Sin B; Celastrol targets adenylyl cyclase-associated protein 1 to reduce macrophages-mediated inflammation and ameliorates high fat diet-induced metabolic syndrome in mice.; Authors - Yuyu Zhu
		Biomaterials; Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.; Authors - Haoran Wang
human	IF	J Immunother Cancer; TSH-TSHR axis promotes tumor immune evasion.; Authors - Zhenghao Wu
		Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang

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