Anticorps Polyclonal de lapin anti-Integrin Beta 1/CD29

• Phare
• Validé par KD/KO

Integrin Beta 1/CD29 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(112)

Avis (6)

N° de cat : 12594-1-AP

Synonymes

ITGB1, CD29, FNRB, Glycoprotein IIa, GPIIA

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Galerie de données de validation

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  WB analysis using 12594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12594-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 12594-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 12594-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 12594-1-AP (Integrin Beta 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 12594-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12594-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12594-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12594-1-AP (Integrin beta-1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 12594-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 12594-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Integrin Beta 1 antibody (12594-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HT-1080, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer du sein humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 75 publications below
• IHC: See 19 publications below
• IF: See 33 publications below
• IP: See 3 publications below

Informations sur le produit

12594-1-AP cible Integrin Beta 1/CD29 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, Lapin, poulet, souris, cerf Sika
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin Beta 1/CD29 Protéine recombinante Ag3078
• Nom complet: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 100-130 kDa
• Numéro d’acquisition GenBank: BC020057
• Symbole du gène: Integrin beta 1
• Identification du gène (NCBI): 3688
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Integrin beta-1 (ITGB1), also named as CD29, is a single chain type I glycoprotein that is expressed in a heterodimeric complex with one of six distinct α subunits, comprising the very late activation antigen (VLA) subfamily of adhesion receptors. It is one of the essential surface molecules expressed on human MSC from bone marrow and other sources. The β1 subunit is also broadly expressed on lymphocytes and monocytes, weakly expressed on granulocytes, and not expressed on erythrocytes. These receptors are involved in a variety of cell-cell and cell-matrix interactions.

Publications

Species	Application	Title
rabbit,human	IF	Small; A Spatiotemporal Controllable Biomimetic Skin for Accelerating Wound Repair; Authors - Yuewei Chen
human	WB	Sci Adv; Decellularized extracellular matrix scaffolds identify full-length collagen VI as a driver of breast cancer cell invasion in obesity and metastasis.; Authors - Andrew L Wishart
human	WB	Nat Commun; Homophilic ATP1A1 binding induces activin A secretion to promote EMT of tumor cells and myofibroblast activation.; Authors - Yi-Ing Chen
human	IF	Sci Adv; Podocyte injury elicits loss and recovery of cellular forces.; Authors - Kathryn E Haley
human	WB	Adv Healthc Mater; Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.; Authors - Moyuan Deng ; KD Validated
human	WB	Sci Bull (Beijing); The Rhinolophus affinis bat ACE2 and multiple animal orthologs are functional receptors for bat coronavirus RaTG13 and SARS-CoV-2.; Authors - Pei Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikita (Verified Customer) (07-23-2025)

I have used it for both ICC and Western blot. I haven't been successful with the blot yet, but my ICC images looked beautiful on embryonic day 17 mouse cortical neurons cultured in vitro for 2 days.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Embryonic mouse cortical neurons

Integrin Beta 1/CD29 Antibody Western Blot,Immunofluorescence validation (1:1000 dilution) in Embryonic mouse cortical neurons (Cat no:12594-1-AP)

FH

Angie (Verified Customer) (08-22-2024)

A549 lysate was subjected to western blot with Integrin beta 1antibody used at dilution of 1:5000 and incubated at room temperature for 1.5 hours. Secondary antibody: Donkey-anti-rabbit (Alexa Fluor 800) at 1:20000 dilution incubated at room temperature for 1 hour. Several bands were observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: A549

Integrin Beta 1/CD29 Antibody Western Blot validation (1:5000 dilution) in A549 (Cat no:12594-1-AP)

FH

Alberto (Verified Customer) (01-16-2024)

Multiple bands, very different compared to advertised picture. I want my money back.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: astrocyte cell line

Integrin Beta 1/CD29 Antibody Western Blot validation (1:500 dilution) in astrocyte cell line (Cat no:12594-1-AP)

FH

Udesh (Verified Customer) (07-10-2022)

The antibody worked great in Co-IP and WB. Great chunky band close to 130 kD. For Co-IP: 6uL/200ug protein and 1:2000 in 3%BSA for WB

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Bone Marrow MSCs

FH

Guorong (Verified Customer) (05-23-2022)

works better in ARPE-19 cells than 293T cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: 293T and ARPE-19

Integrin Beta 1/CD29 Antibody Western Blot validation (1:2000 dilution) in 293T and ARPE-19 (Cat no:12594-1-AP)

FH

Boyan (Verified Customer) (03-29-2019)

Good for WB - recognises a clean band around the expected molecular weight.

• Applications: Western Blot,

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