Anticorps Polyclonal de lapin anti-Centromere protein R

• Phare
• Validé par KD/KO

Centromere protein R Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 10743-1-AP

Synonymes

ITGB3BP, HSU37139, CENP-R, CENPR, CENP R

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Galerie de données de validation

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  WB analysis of K-562 using 10743-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10743-1-AP (Centromere protein R antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10743-1-AP

  IP result of anti-Centromere protein R (IP:10743-1-AP, 4ug; Detection:10743-1-AP 1:300) with K-562 cells lysate 2400 ug.

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  IHC staining of human skin cancer using 10743-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 10743-1-AP (Centromere protein R antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 10743-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using Centromere protein R antibody (10743-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules K-562,
• Résultats positifs en IP: cellules K-562,
• Résultats positifs en IHC: tissu de cancer de la peau humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 4 publications below
• IP: See 3 publications below

Informations sur le produit

10743-1-AP cible Centromere protein R dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Centromere protein R Protéine recombinante Ag1204
• Nom complet: integrin beta 3 binding protein (beta3-endonexin)
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC009929
• Symbole du gène: ITGB3BP
• Identification du gène (NCBI): 23421
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITGB3BP, also named as CENPR and NRIF3; is a transcription coregulator that can have both coactivator and corepressor functions. ITGB3BP acts as a coactivator for estrogen receptor alpha. It acts as a transcriptional corepressor via its interaction with the NFKB1 NF-kappa-B subunit, possibly by interfering with the transactivation domain of NFKB1. ITGB3BP induces apoptosis in breast cancer cells, but not in other cancer cells, via a caspase-2 mediated pathway that involves mitochondrial membrane permeabilization but does not require other caspases. ITGB3BP has five isoforms with MW 7 kDa, 13 kDa and 19-25 kDa.

Publications

Species	Application	Title
human	WB,IF,IP	Cancer Res; Identification of a novel pathway that selectively modulates apoptosis of breast cancer cells.; Authors - Tinnikov Alexander A AA
human	IF,WB,IP	J Mol Cell Biol; Phosphorylation of CENP-R by Aurora B regulates kinetochore-microtubule attachment for accurate chromosome segregation; Authors - Divine Mensah ; KD Validated
human	WB	Oncogene; Serine 28 phosphorylation of NRIF3 confers its co-activator function for estrogen receptor-alpha transactivation.; Authors - Talukder A H AH
human	WB	Stem Cells Transl Med; LncRNA NR_045147 modulates osteogenic differentiation and migration in PDLSCs via ITGB3BP degradation and mitochondrial dysfunction; Authors - Lujue Long
human	IF	J Cell Biol; Interactions with multiple inner kinetochore proteins determine mitotic localization of FACT; Authors - Julia Schweighofer
human	WB,IP,IF	Cell Rep; Condensation-dependent multivalent interactions of EB1 and CENP-R regulate chromosome oscillations in mitosis; Authors - Chengcheng Hu

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