Anticorps Polyclonal de lapin anti-Integrin Beta 7

Integrin Beta 7 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11328-1-AP

Synonymes

Integrin beta 7, integrin, beta 7, ITGB7

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Galerie de données de validation

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  WB analysis of THP-1 using 11328-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 11328-1-AP (Integrin Beta 7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11328-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11328-1-AP (ITGB7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 11328-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11328-1-AP (Integrin Beta 7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 11328-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11328-1-AP (ITGB7 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 11328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11328-1-AP (Integrin Beta 7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 11328-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11328-1-AP (Integrin Beta 7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules THP-1, cellules K-562, tissu pulmonaire humain, tissu splénique de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

11328-1-AP cible Integrin Beta 7 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Integrin Beta 7 Protéine recombinante Ag1871
• Nom complet: integrin, beta 7
• Masse moléculaire calculée: 798 aa, 87 kDa
• Poids moléculaire observé: 87-100 kDa
• Numéro d’acquisition GenBank: BC015916
• Symbole du gène: ITGB7
• Identification du gène (NCBI): 3695
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

ITGB7 (integrin, beta7) is a single-pass type I membrane protein that belongs to the integrin beta chain family. The integrins are a large family of cell-surface glycoprotein receptors that play key roles in leucocyte adhesion, signalling, proliferation and migration by binding as heterodimers to specific ligands. The beta7 integrin associates with alpha4 integrin to form a heterodimer, alpha4/beta7, which interacts with MADCAM1, VCAM, fibronectin, and may also interact with HIV-1 gp120. The beta7 integrin can also form a heterodimer with alphaE integrin, and the dimer, alphaE/beta7 integrin, is a receptor for E-cadherin. ITGB7 is expressed in a variety of leukocyte lines.

Publications

Species	Application	Title
human	IHC	Hum Reprod; Expression of adhesion, attachment and invasion markers in eutopic and ectopic endometrium: a link to the aetiology of endometriosis.; Authors - Sundqvist J J
mouse	IF	J Biol Chem; Multifunctional activity of a small tellurium redox immunomodulator compound, AS101, on DSS-induced murine colitis.; Authors - Gilad Halpert
human	WB	Cell Commun Signal; Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis.; Authors - Tong Sun
human	IF	Cell Mol Gastroenterol Hepatol; Cholinergic anti-inflammatory pathway attenuates Acute Liver Failure through Inhibiting MAdCAM1/α4β7 -Mediated Gut-derived proinflammatory lymphocytes accumulation; Authors - Shan Fu