Anticorps Monoclonal anti-ITK

ITK Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F9D8

N° de cat : 67703-1-PBS

Synonymes

EMT, IL2 inducible T cell kinase, ITK, Kinase EMT, LYK, PSCTK2, T cell specific kinase, Tyrosine protein kinase Lyk

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Galerie de données de validation

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  WB analysis of PBMC using 67703-1-Ig (same clone as 67703-1-PBS)

  PBMC cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67703-1-Ig (ITK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 67703-1-Ig (same clone as 67703-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ITK antibody (67703-1-Ig, Clone: 1F9D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67703-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67703-1-PBS cible ITK dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: ITK Protéine recombinante Ag17087
• Nom complet: IL2-inducible T-cell kinase
• Masse moléculaire calculée: 620 aa, 72 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC109077
• Symbole du gène: ITK
• Identification du gène (NCBI): 3702
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ITK is a member of Tec family (BTK, ITK, Tec, BMX and RLK), which expressed in normal T-lymphocytes and T-cell associated hematopoietic malignancies and have confirmed its critical role in regulating T lymphocyte function in EBV-driven lymphoproliferative disease and immune-mediated disorders. Tec kinase family members shares similarities structure, consisting of PH domain, SH3 domain, SH2 domain and kinase domain. Loss of function mutations in ITK results in hypogammaglobulinemia and CD4+ T cell loss in humans, and the patients often present with EBV-associated B cell lymphoproliferative syndrome (PMID: 30814910, PMID: 31025232).

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