Anticorps Polyclonal de lapin anti-ITPKB

• Phare
• Validé par KD/KO

ITPKB Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12816-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 12816-1-AP (same clone as 12816-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 12816-1-AP (same clone as 12816-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 12816-1-AP (same clone as 12816-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 12816-1-AP (same clone as 12816-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse thymus using 12816-1-AP (same clone as 12816-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12816-1-AP (ITPKB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 12816-1-AP (same clone as 12816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12816-1-AP (same clone as 12816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12816-1-AP (ITPKB Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 12816-1-AP (same clone as 12816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 12816-1-AP (same clone as 12816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 12816-1-AP (ITPKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12816-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12816-1-PBS cible ITPKB dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ITPKB Protéine recombinante Ag3456
• Nom complet: inositol 1,4,5-trisphosphate 3-kinase B
• Masse moléculaire calculée: 946 aa, 102 kDa
• Poids moléculaire observé: 102-110 kDa
• Numéro d’acquisition GenBank: BC015009
• Symbole du gène: ITPKB
• Identification du gène (NCBI): 3707
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Inositol 1,4,5-trisphosphate 3-kinase isoform B (IITPKB) is also named as IP3KB and possesses important roles in the development of immune cells. IP3KB can be targeted to multiple cellular compartments, among them nuclear localization and binding in close proximity to the plasma membrane. The B isoform is the only IP3K that is almost ubiquitously expressed in mammalian cells(PMID:21148483).Three isoforms of IP3KB occur in mammals, which are distinguished by different molecular masses, sensitivity to Ca2+/CaM(calmodulin), intracellular distribution and tissue expression (PMID:16173920). ITPKB can be cleaved into many fragments, this antibody can recognize the N-terminal peptide fragment.(PMID:15130091)

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