Anticorps Polyclonal de lapin anti-ITPKC

ITPKC Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14267-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 14267-1-AP (same clone as 14267-1-PBS)

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 14267-1-AP (same clone as 14267-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 14267-1-AP (ITPKC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 14267-1-AP (same clone as 14267-1-PBS)

  IP result of anti-ITPKC (IP:14267-1-AP, 4ug; Detection:14267-1-AP 1:400) with HepG2 cells lysate 1600ug. This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14267-1-AP (ITPKC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 14267-1-AP (same clone as 14267-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14267-1-AP (ITPKC Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 14267-1-AP (same clone as 14267-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using ITPKC antibody 14267-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14267-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14267-1-PBS cible ITPKC dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ITPKC Protéine recombinante Ag5583
• Nom complet: inositol 1,4,5-trisphosphate 3-kinase C
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 100-105 kDa
• Numéro d’acquisition GenBank: BC060788
• Symbole du gène: ITPKC
• Identification du gène (NCBI): 80271
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

ITPKC(Inositol-trisphosphate 3-kinase C) is also named as IP3KC and belongs to the inositol phosphokinase (IPK) family. It can phosphorylate inositol 2,4,5-triphosphate to inositol 2,4,5,6-tetraphosphate and is relatively weakly activated by the calcium-calmodulin complex. ITPKC also acts as a negative regulator of T-cell activation through the Ca(2+)/NFAT signaling pathway, and the C allele may contribute to immune hyperreactivity in Kawasaki disease(PMID:17160344).

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