Anticorps Monoclonal anti-IVD

IVD Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D12A5

N° de cat : 66032-1-PBS

Synonymes

Isovaleryl-CoA dehydrogenase, mitochondrial, EC:1.3.8.4, EC:1.3.8.1, Butyryl-CoA dehydrogenase, ACAD2

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Galerie de données de validation

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  WB analysis using 66032-1-Ig (same clone as 66032-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66032-1-Ig (same clone as 66032-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  WB analysis of human liver using 66032-1-Ig (same clone as 66032-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66032-1-Ig (same clone as 66032-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66032-1-Ig (IVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66032-1-Ig(IVD antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 66032-1-Ig(IVD antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66032-1-Ig (IVD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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  IF Staining of human liver cancer using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using 66032-1-Ig (IVD antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 66032-1-Ig (same clone as 66032-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using IVD antibody 66032-1-Ig at 1:25 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 66032-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66032-1-PBS cible IVD dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: IVD Protéine recombinante Ag18042
• Nom complet: isovaleryl Coenzyme A dehydrogenase
• Masse moléculaire calculée: 46 kDa
• Poids moléculaire observé: 41-46 kDa
• Numéro d’acquisition GenBank: BC017202
• Symbole du gène: IVD
• Identification du gène (NCBI): 3712
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

IVD(Isovaleryl CoA dehydrogenase, mitochondrial) is a member of the acylCoA dehydrogenase family and is involved in the catabolism of leucine. In size, IVD precursor and mature proteins produced by class I mutants are indistinguishable from their normal counterparts. Class II, III, and IV mutants make IVD precursor proteins 42 kD in size rather than the normal 45 kD(PMID:2063866). It has 2 isoforms produced by alternative splicing.

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