Anticorps Monoclonal anti-Iduronate 2 sulfatase

Iduronate 2 sulfatase Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A3F9

N° de cat : 66112-1-PBS

Synonymes

IDS, 1A3F9, Alpha-L-iduronate sulfate sulfatase, EC:3.1.6.13, Iduronate 2-sulfatase

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Galerie de données de validation

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  WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66112-1-Ig (same clone as 66112-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 66112-1-Ig (same clone as 66112-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 66112-1-Ig (same clone as 66112-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66112-1-Ig (same clone as 66112-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66112-1-Ig (same clone as 66112-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human brain using 66112-1-Ig (same clone as 66112-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 66112-1-Ig (Iduronate 2 sulfatase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66112-1-Ig (same clone as 66112-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66112-1-Ig (Iduronate 2 sulfatase antibody at dilution of 1:50. This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66112-1-Ig (same clone as 66112-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Iduronate 2 sulfatase antibody (66112-1-Ig, Clone: 1A3F9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66112-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66112-1-PBS cible Iduronate 2 sulfatase dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Iduronate 2 sulfatase Protéine recombinante Ag19095
• Nom complet: iduronate 2-sulfatase
• Masse moléculaire calculée: 312aa,35 kDa; 550aa,62 kDa
• Poids moléculaire observé: 76 kDa, 55 kDa
• Numéro d’acquisition GenBank: BC006170
• Symbole du gène: IDS
• Identification du gène (NCBI): 3423
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to 55 and 45 kDa mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).

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