Anticorps Recombinant de lapin anti-IgG Isotype Control

IgG Isotype Control Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

n/a

Applications

WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

Publications(3)

CloneNo.

240953C9

N° de cat : 98136-1-RR

Synonymes

Rabbit IgG, Rabbit IgG Control, Rabbit IgG isotype, Rabbit IgG Isotype Control

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Galerie de données de validation

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  WB analysis using 98136-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 98136-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  SDS-PAGE experiment of antibody using 98136-1-RR

  Purity of 98136-1-RR (IgG Isotype Control antibody) was determined by SDS- polyacrylamide gel electrophoresis. The antibody was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  IP experiment of HepG2 using 98136-1-RR

  IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug.

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  IHC staining of human placenta using 98136-1-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 82719-13-RR (PD-L1/CD274 Recombinant antibody) or unstained (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 98136-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 98136-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:2000 (right) or 84192-3-RR (KI67 Recombinant antibody) (left) (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 98136-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:2000 (right) or 84192-3-RR (KI67 Recombinant antibody) (left) (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 98136-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control.

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  IF Staining of MCF-7 using 98136-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).PCYT1A Recombinant antibody (83100-1-RR) is a positive control.

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  IF Staining of K-562 using 98136-1-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).CD71 Recombinant antibody (81793-2-RR) is a positive control.

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  IF Staining of HeLa using 98136-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A549 using 98136-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using IgG Isotype Control, antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).TMEM53 Recombinant antibody (83554-3-RR) is a positive control.

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  FC experiment of SH-SY5Y using 98136-1-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of MCF-7 using 98136-1-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2ug Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody (98030-1-RR, Clone:240159G8) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of RAW 264.7 using 98136-1-RR

  1x10^6 RAW 264.7 cells were intracellularlly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2ug IGFBP2 Recombinant antibody (84092-3-RR, Clone: 241363D8) and PE-Conjugated Goat Anti-Rabbit IgG (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of human PBMCs using 98136-1-RR

  1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed.

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  FC experiment of HeLa using 98136-1-RR

  1x10^6 HeLa cells were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug Anti-Human MICA Rabbit Recombinant Antibody (98110-1-RR, Clone:240735D5) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were not fixed.

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  FC experiment of mouse splenocytes using 98136-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.2 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or unstain. Cells were not fixed.

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  FC experiment of rat splenocytes using 98136-1-RR

  1x10^6 rat splenocytes cells were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), or 0.2 ug Anti-Rat ICAM-1/CD54 Rabbit Recombinant Antibody (98060-1-RR, Clone:240725C2) and PE-conjugated Goat Anti-Rabbit IgG, or unstain. Cells were not fixed.

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  ChIP experiment of HeLa using 98136-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2A (Lys9) (83041-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en IHC: il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en ChIP-qPCR: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IP: See 3 publications below

Informations sur le produit

98136-1-RR cible IgG Isotype Control dans les applications de WB, IHC, IF/ICC, FC, FC (Intra), IP, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons n/a

• Réactivité: n/a
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Small molecule
• Nom complet: IgG Isotype Control
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

Publications

Species	Application	Title
	IP	Biochim Biophys Acta Mol Basis Dis; Ischemia-reperfusion injury induces ZBP1-dependent PANoptosis in endothelial cells; Authors - Yue Cui
	IP	Autophagy; ATG9 inhibits rickettsia binding to the Host cell surface by blocking the rOmpB-XRCC6/KU70 interaction; Authors - Chen Chen
	IP	Acta Pharmacol Sin; Inhibition of CDC27 O-GlcNAcylation coordinates the antitumor efficacy in multiple myeloma through the autophagy-lysosome pathway; Authors - Hai-Qi Wu

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