Anticorps Recombinant de lapin anti-IgG2a, κ Isotype Control

IgG2a, κ Isotype Control Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

n/a

Applications

FC

Conjugaison

Non conjugué

CloneNo.

252252A1

N° de cat : 98599-1-MR

Synonymes

IgG2a Isotype Control, Mouse IgG2a, IgG2a

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Galerie de données de validation

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  FC experiment of human PBMCs using 98599-1-MR

  1x10^6 human PBMCs were unstained (left, blue in histogram) or surface stained with 0.25 ug Mouse IgG2a, κ Isotype Control RecAb (98599-1-MR, Clone:252252A1) (middle, red in histogram) or 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (65569-1-MR, Clone: OKT3) (right, green in histogram) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of mouse bone marrow cells using 98599-1-MR

  1x10^6 mouse bone marrow cells were unstained (left, blue in histogram) or surface stained with 0.25 ug Mouse IgG2a, κ Isotype Control RecAb (98599-1-MR, Clone:252252A1) (middle, red in histogram) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (right, green in histogram) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of THP-1 using 98599-1-MR

  1x10^6 THP-1 cells were unstained (left, blue in histogram) or surface stained with 0.25 ug Mouse IgG2a, κ Isotype Control RecAb (98599-1-MR, Clone:252252A1) (middle, red in histogram) or 0.25 ug Anti-Human CD64 (10.1) Mouse IgG2a Recombinant Antibody (65572-1-MR, Clone: 10.1) (right, green in histogram) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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  FC experiment of HL-60 using 98599-1-MR

  1x10^6 HL-60 cells were unstained (left, blue in histogram) or surface stained with 0.25 ug Mouse IgG2a, κ Isotype Control RecAb (98599-1-MR, Clone:252252A1) (middle, red in histogram) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (right, green in histogram) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: CMSP humaines, cellules HL-60, cellules médullaires de souris, cellules THP-1

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

98599-1-MR cible IgG2a, κ Isotype Control dans les applications de FC et montre une réactivité avec des échantillons n/a

• Réactivité: n/a
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: unknow
• Nom complet: IgG2a, κ Isotype Control
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide
• Conditions de stockage: Store at 2 - 8°C. Stable for one year after shipment.

Informations générales

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

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