Anticorps Recombinant de lapin anti-IgG2a, κ Isotype Control

IgG2a, κ Isotype Control Recombinant Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

n/a

Applications

FC

Conjugaison

Non conjugué

CloneNo.

252252A1

N° de cat : 98599-1-PBS

Synonymes

IgG2a Isotype Control, Mouse IgG2a, IgG2a

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Galerie de données de validation

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  FC experiment of human PBMCs using 98599-1-MR (same clone as 98599-1-PBS)

  1x10^6 human PBMCs were unstained (left, blue in histogram) or surface stained with 0.25 ug Mouse IgG2a, κ Isotype Control RecAb (98599-1-MR, Clone:252252A1) (middle, red in histogram) or 0.25 ug Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (65569-1-MR, Clone: OKT3) (right, green in histogram) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98599-1-PBS in a different storage buffer formulation.

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  FC experiment of mouse bone marrow cells using 98599-1-MR (same clone as 98599-1-PBS)

  1x10^6 mouse bone marrow cells were unstained (left, blue in histogram) or surface stained with 0.25 ug Mouse IgG2a, κ Isotype Control RecAb (98599-1-MR, Clone:252252A1) (middle, red in histogram) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (right, green in histogram) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98599-1-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 98599-1-MR (same clone as 98599-1-PBS)

  1x10^6 THP-1 cells were unstained (left, blue in histogram) or surface stained with 0.25 ug Mouse IgG2a, κ Isotype Control RecAb (98599-1-MR, Clone:252252A1) (middle, red in histogram) or 0.25 ug Anti-Human CD64 (10.1) Mouse IgG2a Recombinant Antibody (65572-1-MR, Clone: 10.1) (right, green in histogram) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98599-1-PBS in a different storage buffer formulation.

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  FC experiment of HL-60 using 98599-1-MR (same clone as 98599-1-PBS)

  1x10^6 HL-60 cells were unstained (left, blue in histogram) or surface stained with 0.25 ug Mouse IgG2a, κ Isotype Control RecAb (98599-1-MR, Clone:252252A1) (middle, red in histogram) or 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (right, green in histogram) and PE-conjugated Goat Anti-Mouse IgG. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98599-1-PBS in a different storage buffer formulation.

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Informations sur le produit

98599-1-PBS cible IgG2a, κ Isotype Control dans les applications de FC et montre une réactivité avec des échantillons n/a

• Réactivité: n/a
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: unknow
• Nom complet: IgG2a, κ Isotype Control
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

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