Anticorps Polyclonal de lapin anti-IkB Alpha

IkB Alpha Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 51066-1-PBS

Synonymes

IKBA, IκB α, NFKBIA, I kappa B alpha, IkappaBalpha

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Galerie de données de validation

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  WB analysis of PC-3 using 51066-1-AP (same clone as 51066-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 51066-1-AP (same clone as 51066-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 51066-1-AP (same clone as 51066-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 51066-1-AP (same clone as 51066-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 51066-1-AP (same clone as 51066-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 51066-1-AP (IkB alpha antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 51066-1-AP (same clone as 51066-1-PBS)

  IP result of anti-IkB Alpha (IP:51066-1-AP, 4ug; Detection:51066-1-AP 1:1000) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 51066-1-AP (IkB alpha antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 51066-1-AP (same clone as 51066-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51066-1-AP (IkB alpha antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 51066-1-PBS in a different storage buffer formulation.

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Informations sur le produit

51066-1-PBS cible IkB Alpha dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC002601
• Symbole du gène: IkB Alpha
• Identification du gène (NCBI): 4792
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.

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