Anticorps Recombinant de lapin anti-IL-1 beta

IL-1 beta Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241001H8

N° de cat : 84274-1-RR

Synonymes

Il1 beta, Il1b, IL-1,IL1, IL-1 beta,IL 1 beta,IL1beta, IL 1 beta

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Galerie de données de validation

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  WB analysis of NR8383 using 84274-1-RR

  LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 84274-1-RR (Il1 beta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84274-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84274-1-RR against Rat Il1 beta were performed. The affinity constant is 0.734 nM.

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Applications testées

• Résultats positifs en WB: LPS and Brefeldin A treated NR8383 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84274-1-RR cible IL-1 beta dans les applications de WB, ELISA et montre une réactivité avec des échantillons rat

• Réactivité: rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 1 beta
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 31 kDa
• Numéro d’acquisition GenBank: NM_031512
• Symbole du gène: IL-1 beta
• Identification du gène (NCBI): 24494
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets.

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