Anticorps Recombinant de lapin anti-IL-10

IL-10 Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

240430D8

N° de cat : 82191-7-PBS

Synonymes

IL-10,IL10, Il10, interleukin 10, Il 10, CSIF

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Galerie de données de validation

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  Sandwich ELISA standard curve of MP00507-4

  Sandwich ELISA standard curve of MP00507-4, IL-10 Recombinant Matched Antibody Pair - PBS only. 82191-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0026.82191-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

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  Sample test of MP00507-4

  Mouse splenocytes (1 x 10^cells/mL) were cultured for 4 days in RPMI supplemented with 10% fetal calf serum, 100 ng/mL of recombinant mouse IFN-γ and 1 μg/mL of LPS. The mean IL-10 concentration was determined to be 7.59 pg/mL in unstimulated spleen cell culture supernatant, 2,461.0 pg/mL in IFN-γ and LPS stimulated spleen cell culture supernatant. Mouse splenocytes (1 x 10^cells/mL) were cultured for 3 days in RPMI supplemented with 10% fetal calf serum,1 μg/mL of LPS. The mean IL-10 concentration was determined to be 11.30 pg/mL in unstimulated spleen cell culture supernatant, 143.99 pg/mL in LPS stimulated spleen cell culture supernatant.

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Informations sur le produit

82191-7-PBS cible IL-10 dans les applications de Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: interleukin 10
• Masse moléculaire calculée: 21 kDa
• Numéro d’acquisition GenBank: NM_010548
• Symbole du gène: Il10
• Identification du gène (NCBI): 16153
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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