Anticorps Recombinant de lapin anti-IL-15RA

IL-15RA Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242618H6

N° de cat : 85671-2-PBS

Synonymes

CD215, IL-15 receptor subunit alpha, Il15ra, IL-15R-alpha, Interleukin-15 receptor subunit alpha

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Galerie de données de validation

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  WB analysis using 85671-2-RR (same clone as 85671-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85671-2-RR (Il15ra antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85671-2-PBS in a different storage buffer formulation.

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  WB analysis using 85671-2-RR (same clone as 85671-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85671-2-RR (Il15ra antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85671-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02016-3

  Sandwich ELISA standard curve of MP02016-3, Mouse IL-15RA Recombinant Matched Antibody Pair - PBS only. 85671-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2687. 85671-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02016-3

  The mean IL-15RA concentration was determined to be 1,332.06 ng/mL in mouse liver tissue extract based on a 3.7 mg/mL extract load.

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Informations sur le produit

85671-2-PBS cible IL-15RA dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-15RA Protéine recombinante Eg2687
• Nom complet: interleukin 15 receptor, alpha chain
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: NM_008358.2
• Symbole du gène: Il15ra
• Identification du gène (NCBI): 16169
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).

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