Anticorps Polyclonal de lapin anti-IL-17a

IL-17a Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 26163-1-AP

Synonymes

Ctla 8, Ctla8, Il17, Il17a, Il-17a, interleukin 17A

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Galerie de données de validation

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  WB analysis using 26163-1-AP

  PMA and ionomycin treated EL-4 cells were subjected to SDS PAGE followed by western blot with 26163-1-AP (IL-17a antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26163-1-AP (Il17a Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26163-1-AP (Il17a Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 26163-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26163-1-AP (IL-17a antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 26163-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using 26163-1-AP (Il17a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse spleen using 26163-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using 26163-1-AP (Il17a antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: PMA, Ionomycin and Brefeldin A treated EL-4 cells,
• Résultats positifs en IHC: tissu splénique de souris, tissu de cancer de l'estomac humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu splénique de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 8 publications below
• IF: See 5 publications below

Informations sur le produit

26163-1-AP cible IL-17a dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-17a Protéine recombinante Ag24087
• Nom complet: interleukin 17A
• Numéro d’acquisition GenBank: NM-010552
• Symbole du gène: Il17a
• Identification du gène (NCBI): 16171
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The IL17A monomer is a peptide consisting of 155 amino acids. The IL17A peptide comprises a 23 amino acid signal peptide and a 132 amino acid mature peptide. The IL17A homodimer has a molecular weight of 35 kD (Kolls and Lindén, 2004).

Publications

Species	Application	Title
mouse	IHC	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang
human	WB	Cell Biol Toxicol; Interleukin-17A mediates tobacco smoke-induced lung cancer epithelial-mesenchymal transition through transcriptional regulation of ΔNp63α on miR-19.; Authors - Chunfeng Xie
human,mouse	IHC	J Cell Mol Med; Ozone therapy promotes the differentiation of basal keratinocytes via increasing Tp63-mediated transcription of KRT10 to improve psoriasis.; Authors - Lihua Gao
mouse	IHC	Biochim Biophys Acta Mol Cell Res; NEK2 overexpression aggravates IL-22-induced keratinocyte proliferation and cytokine level increases and IMQ-induced psoriasis-like dermatitis; Authors - Youhua Peng
mouse	WB	Front Immunol; Critical Role of AdipoR1 in Regulating Th17 Cell Differentiation Through Modulation of HIF-1α-Dependent Glycolysis.; Authors - Qian Zhang
mouse	WB	Front Immunol; IL-17 Aggravates Pseudomonas aeruginosa Airway Infection in Acute Exacerbations of Chronic Obstructive Pulmonary Disease.; Authors - Fengming Ding