Anticorps Recombinant de lapin anti-IL-28A

IL-28A Recombinant Antibody for FC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

FC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242247E2

N° de cat : 98388-1-PBS

Synonymes

Ifnl2, IFN-lambda-2, Il28a, Interferon lambda-2, Interleukin-28A

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Galerie de données de validation

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  FC experiment of Transfected HEK-293T using 98388-1-RR (same clone as 98388-1-PBS)

  1x10^6 HEK-293T cells(left) and Il28a-transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse IL-28A Rabbit RecAb (98388-1-RR, Clone:242247E2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98388-1-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98388-1-RR (same clone as 98388-1-PBS)

  1x10^6 HEK-293T cells(left) and Il28a-transfected HEK-293T cells(right) were stained with 0.25 ug Anti-Mouse IL-28A Rabbit RecAb (98388-1-RR, Clone:242247E2)(red), or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and APC-Conjugated Goat Anti-Rabbit IgG(H+L).Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98388-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01988-2

  Cytometric bead array standard curve of MP01988-2, MOUSE IL-28A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98388-1-PBS. Detection antibody: 85590-3-PBS. Standard: Eg3169. Range: 0.313-20 ng/mL.

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  Affinity and Kinetic Characterization of 98388-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98388-1-RR against Mouse IL-28A were performed. The affinity constant is 0.137 nM.

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Informations sur le produit

98388-1-PBS cible IL-28A dans les applications de FC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: IL-28A Protéine recombinante Eg3169
• Nom complet: interleukin 28A
• Masse moléculaire calculée: 22 kDa
• Numéro d’acquisition GenBank: NM_001024673.2
• Symbole du gène: Il28a
• Identification du gène (NCBI): 330496
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C.

Informations générales

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.

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