Anticorps Polyclonal de lapin anti-IL-6

IL-6 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (3)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(44)

N° de cat : 26404-1-AP

Synonymes

Il 6, Il6, Il-6, interleukin 6

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Galerie de données de validation

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  WB analysis using 26404-1-AP

  untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26404-1-AP (IL-6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules RAW 264.7 traitées au LPS et à la bréfeldine A,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 29 publications below
• IHC: See 11 publications below
• IF: See 7 publications below
• ELISA: See 1 publications below

Informations sur le produit

26404-1-AP cible IL-6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, bovin, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-6 Protéine recombinante Ag24800
• Nom complet: interleukin 6
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: NM_031168
• Symbole du gène: Il6
• Identification du gène (NCBI): 16193
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Publications

Species	Application	Title
mouse	WB	Nat Aging; Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer; Authors - Yifei Cheng
mouse	WB	J Nanobiotechnology; Dental pulp mesenchymal stem cell-derived exosomes inhibit neuroinflammation and microglial pyroptosis in subarachnoid hemorrhage via the miRNA-197-3p/FOXO3 axis; Authors - Xin Liang
mouse	WB,IHC	Sci Total Environ; Neurotoxic effects of triclosan in adolescent mice: Pyruvate kinase M2 dimer regulated signal transducer and activator of transcription 3 phosphorylation mediated microglia activation and neuroinflammation; Authors - Ziyun Wei
rat	IF	Int J Biol Macromol; Injectable hyaluronate-based hydrogel with a dynamic/covalent dual-crosslinked architecture for bone tissue engineering: Enhancing osteogenesis and immune regulation; Authors - Kunyao Guo
mouse	WB	Nutrients; Luteolin Alleviates Ulcerative Colitis in Mice by Modulating Gut Microbiota and Plasma Metabolism; Authors - Shuai Yang
mouse	IHC	Int Immunopharmacol; Bomidin attenuates inflammation of periodontal ligament stem cells and periodontitis in mice via inhibiting ferroptosis; Authors - Wei Wu

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