Anticorps Polyclonal de lapin anti-IL-6

IL-6 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 26404-1-AP

Synonymes

Il 6, Il6, Il-6, interleukin 6

---

Galerie de données de validation

• 

  WB analysis using 26404-1-AP

  untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 26404-1-AP (IL-6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

---

Applications testées

• Résultats positifs en WB: cellules RAW 264.7 traitées au LPS et à la bréfeldine A,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 2 publications below
• ELISA: See 1 publications below

Informations sur le produit

26404-1-AP cible IL-6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IL-6 Protéine recombinante Ag24800
• Nom complet: interleukin 6
• Masse moléculaire calculée: 24 kDa
• Poids moléculaire observé: 24 kDa
• Numéro d’acquisition GenBank: NM_031168
• Symbole du gène: Il6
• Identification du gène (NCBI): 16193
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Publications

Species	Application	Title
mouse	IHC	Int Immunopharmacol; Bomidin attenuates inflammation of periodontal ligament stem cells and periodontitis in mice via inhibiting ferroptosis; Authors - Wei Wu
	WB	J Transl Med; Immunotherapy landscape analyses of necroptosis characteristics for breast cancer patients.; Authors - Honghao Yu
mouse	WB	BMC Complement Med Ther; Ethanol extract of Paridis rhizoma attenuates carrageenan-induced paw swelling in rats by inhibiting the production of inflammatory factors; Authors - Li Xiang
mouse	WB	Cytokine; Schaftoside reduces inflammation in Aspergillus fumigatus keratitis through the inhibition of the TLR4/MyD88 pathway; Authors - Danli Lu
mouse	WB	Antioxidants (Basel); Extracellular Superoxide Dismutase Attenuates Hepatic Oxidative Stress in Nonalcoholic Fatty Liver Disease through the Adenosine Monophosphate-Activated Protein Kinase Activation; Authors - Heechul Nam
mouse	ELISA	Biomed Res Int; Mechanical Stretch Promotes Macrophage Polarization and Inflammation via the RhoA-ROCK/NF-κB Pathway; Authors - Peng-cheng Tu