Anticorps Recombinant de lapin anti-CD126/IL-6R alpha

CD126/IL-6R alpha Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris

Applications

Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230371E7

N° de cat : 83209-2-PBS

Synonymes

CD126, Il6ra, IL 6R, Il6r, IL-6R alpha

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Galerie de données de validation

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  Sandwich ELISA sample test of MP00182-2

  J774A.1 mouse reticulum cell sarcoma macrophage cells (1 x 106 cells/mL) were cultured for 3 days in DMEM containing 10% fetal bovine serum. An aliquot of the cell culture supernatants were removed, assayed for mouse IL-6R alpha, and measured 402.5 pg/mL. Liver tissue from one mouse was homogenized and seeded into 100 mL of RPMI containing 10% fetal bovine serum, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and 50 μM β-mercaptoethanol for 3 days. An aliquot of the cell culture supernate was removed, assayed for mouse IL-6R alpha, and measured 345.1 pg/mL.

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  Sandwich ELISA sample test of MP00182-2

  Serum of sixteen mice were measured. The IL-6R alpha concentration of detected samples was determined to be 10,180.3 pg/mL with a range of 6,065.2-15,362.2 pg/mL.

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  Sandwich ELISA standard curve of MP00182-2

  Sandwich ELISA standard curve of MP00182-2, Mouse CD126/IL-6R alpha Monoclonal Matched Antibody Pair - PBS only. 98024-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0664. 83209-2-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL.

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Informations sur le produit

83209-2-PBS cible CD126/IL-6R alpha dans les applications de Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CD126/IL-6R alpha Protéine recombinante Eg0664
• Nom complet: interleukin 6 receptor, alpha
• Masse moléculaire calculée: 50KD
• Numéro d’acquisition GenBank: NM_010559.3
• Symbole du gène: Il6ra
• Identification du gène (NCBI): 16194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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