Anticorps Monoclonal anti-Importin Beta

Importin Beta Monoclonal Antibody for WB, IHC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain

Applications

WB, IHC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1A12B1

N° de cat : 67597-1-PBS

Synonymes

KPNB1, Importin subunit beta-1, Importin subunit beta 1, Importin 90, Impnb

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Galerie de données de validation

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  WB analysis using 67597-1-Ig (same clone as 67597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67597-1-Ig (Importin beta antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

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  WB analysis using 67597-1-Ig (same clone as 67597-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67597-1-Ig (Importin Beta antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67597-1-Ig (same clone as 67597-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67597-1-Ig (Importin beta antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 67597-1-Ig (same clone as 67597-1-PBS)

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human Importin Beta (67597-1-Ig, Clone:1A12B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 67597-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67597-1-PBS cible Importin Beta dans les applications de WB, IHC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Importin Beta Protéine recombinante Ag23094
• Nom complet: karyopherin (importin) beta 1
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 95-97 kDa
• Numéro d’acquisition GenBank: BC003572
• Symbole du gène: KPNB1
• Identification du gène (NCBI): 3837
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Importin beta1, also known karyopherin beta or KPNB1, is a nuclear transport receptor that plays a key role in the nuclear import process. Importin beta1 and importin alpha work in concert to transport cargo into the nucleus, functioning as an essential component of the classical nuclear localization signal (NLS) pathway. Elevated expression of importin beta1 has been found in cervical tumors. (22125623)

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